Journal: International Journal of Nanomedicine

Article Title: Sinoporphyrin sodium, a novel sensitizer, triggers mitochondrial-dependent apoptosis in ECA-109 cells via production of reactive oxygen species

doi: 10.2147/ijn.s59302

Figure Lengend Snippet: Figure 12 Activation of apoptosis and oxidative stress-related proteins in ECA-109 cells posttreatment. Cleavage of caspase-3 and phospho-p38 was detected by Western blotting analysis, with β-actin used as a loading control. The ratios of cleaved caspase-3 and phospho-p38 were analyzed by Quantity One software. Control, untreated cells; S3, cells treated with 4 μM DVDMS plus 3 minutes of ultrasound irradiation; S3 + N-acetylcysteine, cells treated with 4 μM DVDMS plus 3 minutes of ultrasound irradiation in the presence of N-acetylcysteine. Data are presented as the mean ± standard deviation of three independent assessments. *P,0.05 and **P,0.01 versus untreated control. Abbreviations: NAC, N-acetylcysteine; p-p38, phospho-p38.

Article Snippet: After washing and blocking, the membranes were incubated overnight at 4°C with primary antibodies against rabbit polyclonal caspase-3 (Cell Signaling Technology, Danvers, MA, USA) and rabbit polyclonal phospho-p38 mitogenactivated protein kinase (MAPK, Cell Signaling Technology).

Techniques: Activation Assay, Western Blot, Control, Software, Irradiation, Standard Deviation

Journal: Thrombosis research

Article Title: Carbon monoxide (CO)-releasing molecule-derived CO regulates tissue factor and plasminogen activator inhibitor type 1 in human endothelial cells.

doi: 10.1016/j.thromres.2012.07.002

Figure Lengend Snippet: Fig. 3. Effects of pretreatment with CORM-2 on TNF-α stimulated mitogen-activated protein kinases (MAPKs) phosphorylation. HUVECs were pretreated with CORM-2 at a concentration of 50 μM for 3 h, and stimulated with 10 ng/ml TNF-α for additional 0–1 h. Phosphorylations of p38 MAPK, ERK1/2, and JNK were analyzed by western blots. A representative image from three experiments is shown.

Article Snippet: Anti-TF, Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), p38 mitogenactivated protein kinase (p38 MAPK), extracellular signal-regulated kinase1/2 (ERK1/2), c-Jun N-terminal kinase (JNK), phosho-p38 MAPK, phospho-ERK1/2, phospho-JNK, nuclear factor-kappa B (NF-κB, p65), IκBα, and Oct-1 polyclonal antibodies were purchased from Santa Cruz Biotechnology Inc (CA, USA).

Techniques: Phospho-proteomics, Concentration Assay, Western Blot

Journal: Thrombosis research

Article Title: Carbon monoxide (CO)-releasing molecule-derived CO regulates tissue factor and plasminogen activator inhibitor type 1 in human endothelial cells.

doi: 10.1016/j.thromres.2012.07.002

Figure Lengend Snippet: Fig. 4. Effects of MAPK inhibitors on TF and PAI-1 protein expression in TNF-α-stimulated HUVECs. HUVECs were pre-incubated with p38 MAPK inhibitor SB203580 (20 μM), ERK1/2 inhibitor U0126 (20 μM), or JNK inhibitor SP600125 (20 μM) for 1 h and then stimulated with TNF-α for 5 h. TF and PAI-1 protein expres- sion were analyzed by western blots. A representative image from three experiments is shown. Control: unstimulated cells.

Article Snippet: Anti-TF, Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), p38 mitogenactivated protein kinase (p38 MAPK), extracellular signal-regulated kinase1/2 (ERK1/2), c-Jun N-terminal kinase (JNK), phosho-p38 MAPK, phospho-ERK1/2, phospho-JNK, nuclear factor-kappa B (NF-κB, p65), IκBα, and Oct-1 polyclonal antibodies were purchased from Santa Cruz Biotechnology Inc (CA, USA).

Techniques: Expressing, Incubation, Western Blot, Control